{"id":15420,"date":"2026-06-24T13:16:19","date_gmt":"2026-06-24T12:16:19","guid":{"rendered":"https:\/\/izvestiyakbncran.ru\/?page_id=15420"},"modified":"2026-06-30T12:36:25","modified_gmt":"2026-06-30T11:36:25","slug":"28-3-11-en","status":"publish","type":"page","link":"https:\/\/izvestiyakbncran.ru\/index.php\/en\/28-3-11-en\/","title":{"rendered":"28.3.11 En"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-lora-font-family\" style=\"font-size:22px\"><strong>Impact of Lignohumate antidote application timing on the amino acid composition of winter wheat grain during chemical weeding<\/strong><\/h2>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-1 wp-block-paragraph\" style=\"margin-top:0;margin-bottom:0;padding-top:0;padding-bottom:0\"><strong>L.A. Alakaeva, A.Kh. Zanilov, M.R. Aznaeva, A.M. Leshkenov, T.B. Shalov<\/strong><\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-content-justification-left is-nowrap is-layout-flex wp-container-core-group-is-layout-20193d73 wp-block-group-is-layout-flex\" style=\"border-style:none;border-width:0px;margin-top:0;margin-bottom:0;padding-top:0;padding-bottom:0\">\n<p class=\"has-text-color has-link-color has-lora-font-family wp-elements-2 wp-block-paragraph\" style=\"color:#5b1919;font-size:12px;text-decoration:underline\"><\/p>\n\n\n\n<div class=\"wp-block-group is-horizontal is-layout-flex wp-container-core-group-is-layout-9076828a wp-block-group-is-layout-flex\" style=\"min-height:0px;margin-top:0;margin-bottom:0;padding-top:0;padding-bottom:0\">\n<div class=\"wp-block-buttons is-content-justification-left is-layout-flex wp-container-core-buttons-is-layout-856cf56e wp-block-buttons-is-layout-flex\" style=\"margin-top:0;margin-bottom:0;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-100 is-style-outline is-style-outline--1\"><a class=\"wp-block-button__link has-background-background-color has-text-color has-background has-link-color has-border-color has-custom-font-size wp-element-button\" href=\"http:\/\/izvestiyakbncran.ru\/wp-content\/uploads\/2026\/06\/11.-alakaeva-zanilov-aznaeva.pdf\" style=\"border-color:#5b1919;border-style:solid;border-width:2px;border-radius:8px;color:#5b1919;padding-top:0.4rem;padding-right:var(--wp--preset--spacing--40);padding-bottom:0.4rem;padding-left:var(--wp--preset--spacing--40);font-size:12px\">PDF<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:0px;width:0px\" aria-hidden=\"true\" class=\"wp-block-spacer wp-container-content-273e683f\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-100 is-style-outline is-style-outline--2\"><a class=\"wp-block-button__link has-background-background-color has-text-color has-background has-link-color has-border-color has-text-align-center has-custom-font-size wp-element-button\" href=\"http:\/\/izvestiyakbncran.ru\/wp-content\/uploads\/2026\/06\/11-impact.xml\" style=\"border-color:#5b1919;border-width:2px;border-top-left-radius:8px;border-top-right-radius:8px;border-bottom-left-radius:8px;border-bottom-right-radius:8px;color:#5b1919;padding-top:0.4rem;padding-right:var(--wp--preset--spacing--40);padding-bottom:0.4rem;padding-left:var(--wp--preset--spacing--40);font-size:12px\">JATS XML<\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-3 wp-block-paragraph\" style=\"border-style:none;border-width:0px;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-left-radius:0px;border-bottom-right-radius:0px;color:#5b1919;margin-top:0;margin-right:0;margin-bottom:0;margin-left:0;padding-top:0;padding-right:0;padding-bottom:0;padding-left:0\"><\/p>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\"\/>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-4 wp-block-paragraph\" style=\"line-height:1.4\"><em><strong><strong>Abstract<\/strong><\/strong>. <\/em>In the context of intensive farming, mitigating the phytotoxic effect of herbicides while maintaining and improving the quality of crop products remains an urgent task. Of particular interest is the use of humic-nature antidotes, which are capable of exerting an anti-stress effect and influencing the biochemical composition of grain. At the same time, no data are available regarding how the timing and sequence of antidote application during herbicide treatments affect grain quality under the conditions of the Kabardino-Balkarian Republic steppe zone.<br><strong>Aim<\/strong>. The goal of the study is to assess how the timing of Lignohumate AM antidote application (150 g\/ha) during herbicide treatments affects the amino acid profile of winter wheat grain and overall crop yield. Thus, the novelty of this study is that it evaluates the effects of antidotes on both crop yield and grain nutritional density (essential amino acid concentration) as a function of their application sequencing relative to herbicides.<br><strong>Materials and methods<\/strong>. Field trials were conducted on winter wheat crops of the &#8216;Pamyati Shatilova&#8217; variety on the experimental field of the Institute of Agriculture of the Kabardino-Balkarian Scientific Center of the Russian Academy of Sciences (IA KBSC RAS). The treatment of winter wheat crops was carried out during the spring tillering phase according to the following scheme: 1) 2 days before chemical weeding; 2) in a tank mix with the herbicide; 3) 2 days after chemical weeding. The herbicide &#171;Ballerina&#187; served as the plant protection product. High-performance liquid chromatography was then used to determine the content of essential amino acids. Statistical analysis of the results involved ANOVA and Tukey&#8217;s test at p &lt; 0.05.<\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-5 wp-block-paragraph\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20);line-height:1.4\"><strong>Results<\/strong>. The study found that the maximum grain yield (5.27 t\/ha) was achieved by applying the antidote in a tank mixture, which exceeded the control (4.75 t\/ha) by 10.9 %. The highest total concentration of essential amino acids among the treatments was also observed in the tank mix option (3.641 %), which was 7.8 % lower than that in the control (3.949%). The lowest efficacy was observed with the preliminary antidote application (2.642 %). Meanwhile, the crop yield trended downward within the margin of statistical error (2.7 %).<br><strong>Conclusion<\/strong>. The obtained results indicate the feasibility of applying Lignohumate in a tank mixture with the herbicide as the most effective way to realize its anti-stress and biofortification potential.<\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-6 wp-block-paragraph\" style=\"line-height:1.4\"><\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-7 wp-block-paragraph\" style=\"line-height:1.4\"><strong><em><strong>Keywords<\/strong><\/em><\/strong><em>:<\/em> winter wheat, Lignohumate, antidote, herbicide, chemical weed control, amino acid composition, essential amino acids, biofortification, phytotoxicity<\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-8 wp-block-paragraph\" style=\"line-height:1.4\"><\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-9 wp-block-paragraph\" style=\"font-size:12px;line-height:1.4\"><strong><strong>For citation<\/strong>.<\/strong> Alakaeva L.A., Zanilov A.Kh., Aznaeva M.R., Leshkenov A.M., Shalov T.B. Impact of Lignohumate antidote application timing on the amino acid composition of winter wheat grain during chemical weeding. News of the Kabardino-Balkarian Scientific Center of RAS. 2026. Vol. 28. No. 3. Pp. 143\u2013152. DOI: 10.35330\/1991-6639-2026-28-3-143-152<\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-10 wp-block-paragraph\" style=\"font-size:12px;line-height:1.4\"><\/p>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-11 wp-block-paragraph\" style=\"font-size:12px;line-height:1.4\">\u00a9&nbsp;&nbsp; Alakaeva L.A., Zanilov A.Kh., Aznaeva M.R., Leshkenov A.M., Shalov T.B., 2026<\/p>\n\n\n\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-8e2b6fff wp-block-group-is-layout-flex\" style=\"margin-top:var(--wp--preset--spacing--20);padding-top:0;padding-bottom:0\">\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"80\" height=\"28\" src=\"https:\/\/izvestiyakbncran.ru\/wp-content\/uploads\/2026\/03\/image.png\" alt=\"\" class=\"wp-image-7229\"\/><\/figure>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-12 wp-block-paragraph\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:0;font-size:12px\">Content is available under license&nbsp;<a href=\"http:\/\/creativecommons.org\/licenses\/by\/4.0\/\" target=\"_blank\" rel=\"noreferrer noopener\">Creative Commons Attribution 4.0 License<\/a><\/p>\n<\/div>\n\n\n\n<p class=\"has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-13 wp-block-paragraph\" style=\"font-size:12px;line-height:1.4\"><\/p>\n\n\n\n<details class=\"wp-block-details has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-14 is-layout-flow wp-container-core-details-is-layout-f488f964 wp-block-details-is-layout-flow\" style=\"font-style:normal;font-weight:700;line-height:1.5\"><summary><strong>R<\/strong>eferences<\/summary>\n<ol style=\"margin-top:0;margin-bottom:0\" class=\"wp-block-list\">\n<li style=\"font-style:normal;font-weight:400\">Kostin Ya.V., Ushakov R.N., Kryuchkov M.M. et al. Agroecological efficiency of Extrasol biopreparate for barley growing. Bulletin of the Ryazan State Agrotechnological University named after P.A. Kostychev. 2017. No. 3(35). Pp. 34\u201338. EDN: ZHMYAB. (Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Nakaryakov A.M., Zavalin A.A. Influence of biorperarates and fertilizers on the yield and quality of winter wheat grain on light gray forest soil. Plodorodie. 2021. No. 4(121). Pp. 26\u201330. DOI: 10.25680\/S19948603.2021.121.08. (Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Kishev A.Yu., Khanieva I.M., Berbekov K.Z. Application of lignohumate and albite in combination with nitrogen fertilization on sugar beet. Izvestiya of Kabardino-Balkarian<br>State Agrarian University named after V.M. Kokov. 2020. No. 3(29). Pp. 7\u201310. EDN: ZGUXAJ. (in Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Pozdnyakov L.A., Stepanov A.L., Gasanov M.E. et al. Effect of lignohumate on soil biological activity on the Bali Island, Indonesia. Pochvovedenie. 2020. No. 5. Pp. 601\u2013609. DOI:<br>10.31857\/S0032180X20050111. EDN XWVBTQ. (Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Bychkova V.V., Sazonova I.A., Pidenko P.S. et al. Negative effects of imidazolinone herbicides usage: problems and decisions. Agrohimia. 2023. No. 2. Pp. 87\u201396. DOI: 10.31857\/S0002188123020060. EDN: MSAXSU. (in Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Dvoryankin E.A. Crop losses due to herbicide phytotoxicity. Methods for studying herbicide toxicity. Sakhar. 2018. No. 7. Pp. 25\u201329. EDN: UWRPMC. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Kravchenko R.V., Radchevskiy P.P., Prakh A.V. Quality of Saperavi grapes and wine when applying the lignohumates of &#171;A&#187; grade. Polythematic Online Scientific Journal of Kuban State Agrarian University. 2014. No. 99. Pp. 749\u2013759. EDN: SGTOMP. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Pashkevich E.B., Larina G.E., Parakhina M.V. Influence of different methods of application of ugnogumate on the product quality of green crops and medicinal plants. Agrohimia. 2024. No. 4. Pp. 60\u201368. DOI: 10.31857\/S0002188124040079. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Kondratenko E.P., Konstantinova O.B., Soboleva O.M. et al. The content of protein and amino acids in the grain of winter crops growing in the forest-steppe of the south-east of<br>Western Siberia. Khimiya rastitel&#8217;nogo syr&#8217;ya. 2015. No. 3. Pp. 143\u2013150. EDN: VEJQMH. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Korshunov A.V., Mityshkin A.V., Gaitova N.A. et al. Effectiveness of lignin-humates and complex fertilizer akvarin-12 on potato. Achievements of Science and Technology in Agro-Industrial Complex. 2009. No. 11. Pp. 17\u201319. EDN: MEGJFD. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Sheudzhen A.Kh., Gutorova O.A., Lutsenko I.M. Agrochemical estimation of lignogumate on winter wheat crops in the conditions of the NorthWestern Precaucasia. Agrohimia. 2022. No. 3. Pp. 31\u201340. DOI: 10.31857\/S0002188122030103. EDN: RVMNEQ. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Boyko V.A., Levchenko S.V., Belash D.Yu., Romanov A.V. Impact of \u201cLignohumate\u201d fertilizer on productivity indicators and grapes quality in the conditions of the Republic of Crimea. Russkiy vinograd [Russian Grapes]. 2021. Vol. 15. Pp. 43\u201351. DOI: 10.32904\/2712-8245-2021-15-43-51. EDN: GFOKXI. (In Russian)<\/li>\n\n\n\n<li style=\"font-style:normal;font-weight:400\">Rudenko A.O., Kartsova L.A., Snarsky S.I. High performance liquid chromatography determination of the major amino acids in complex biological objects using phenylisothiocyanate derivatization. Sorption and Chromatography Processes. 2010. Vol. 10. No. 2. Pp. 223\u2013230. EDN: MUEQTX. (In Russian)<\/li>\n<\/ol>\n<\/details>\n\n\n\n<details class=\"wp-block-details has-foreground-color has-text-color has-link-color has-lora-font-family has-extra-small-font-size wp-elements-15 is-layout-flow wp-container-core-details-is-layout-9ff6af70 wp-block-details-is-layout-flow\" style=\"font-style:normal;font-weight:700;line-height:1.5\"><summary><strong>Information about the authors<\/strong><\/summary>\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-1c18c512 wp-block-group-is-layout-flex\" style=\"min-height:0px;margin-top:0;margin-bottom:0;padding-top:var(--wp--preset--spacing--20);padding-right:var(--wp--preset--spacing--40);padding-bottom:var(--wp--preset--spacing--20);padding-left:var(--wp--preset--spacing--40)\">\n<p class=\"wp-block-paragraph\" style=\"font-style:normal;font-weight:400\"><strong>Leila A. Alakaeva<\/strong>, Postgraduate Student, Scientific and Educational Center, Head of the Department of Program Development and Contractual Activities, Kabardino-Balkarian Scientific Center of the Russian Academy of Sciences;<br>2, Balkarov street, Nalchik, 360010, Russia; <br><strong>Amiran Kh. Zanilov<\/strong>, Candidate of Agricultural Sciences, Senior Researcher, Center for Decarbonization of the Agro-Industrial Complex and Regional Economy, Kabardino-Balkarian State University named after Kh.M. Berbekov;<br>173, Chernyshevsky street, Nalchik, 360004, Russia;<br>amiran78@inbox.ru, ORCID: https:\/\/orcid.org\/0009-0002-8635-6501<br><strong>Milana R. Aznaeva<\/strong>, Head of the Department for Monitoring Agroecosystems, Center for Decarbonization of the Agro-Industrial Complex and Regional Economy, Kabardino-Balkarian State University named after Kh.M. Berbekov;<br>173, Chernyshevsky street, Nalchik, 360004, Russia;<br>miazn@mail.ru, ORCID: https:\/\/orcid.org\/0009-0000-9327-4526<br><strong>Aslan M. Leshkenov<\/strong>, Head of the Laboratory of Agrochemistry and Soil Research, Institute of Agriculture \u2013 branch of the Kabardino-Balkarian Scientific Center of the Russian Academy of Sciences;<br>224, Kirov street, Nalchik, 360004, Russia;<br>aslan.leshckenov@yandex.ru, ORCID: https:\/\/orcid.org\/0000-0001-9516-3213<br><strong>Timur B. Shalov<\/strong>, Doctor of Agricultural Sciences, Professor, Head of the Department of Science and Postgraduate Studies, Abkhazian State University;<br>1, Universitetskaya street, Sukhum, 384904, Republic of Abkhazia;<br>timur.shalov@mail.ru, ORCID: https:\/\/orcid.org\/0000-0002-7015-9266<\/p>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-style:normal;font-weight:400\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-style:normal;font-weight:400\"><\/p>\n<\/div>\n<\/details>\n\n\n\n<details class=\"wp-block-details has-foreground-color has-text-color has-link-color has-lora-font-family wp-elements-16 is-layout-flow wp-block-details-is-layout-flow\" style=\"font-size:14px\"><summary><strong>Funding<\/strong><\/summary>\n<p class=\"wp-block-paragraph\" style=\"margin-top:var(--wp--preset--spacing--20);margin-bottom:var(--wp--preset--spacing--20)\">The study was performed without external funding.<\/p>\n<\/details>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Impact of Lignohumate antidote application timing on the amino acid composition of winter wheat grain during chemical weeding L.A. Alakaeva, A.Kh. Zanilov, M.R. Aznaeva, A.M. Leshkenov, T.B. Shalov Abstract. In the context of intensive farming, mitigating the phytotoxic effect of herbicides while maintaining and improving the quality of crop products remains an urgent task. Of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"wp-custom-template-home","meta":{"footnotes":""},"class_list":["post-15420","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>28.3.11 En - \u0418\u0417\u0412\u0415\u0421\u0422\u0418\u042f \u041a\u0410\u0411\u0410\u0420\u0414\u0418\u041d\u041e-\u0411\u0410\u041b\u041a\u0410\u0420\u0421\u041a\u041e\u0413\u041e \u041d\u0410\u0423\u0427\u041d\u041e\u0413\u041e \u0426\u0415\u041d\u0422\u0420\u0410 \u0420\u0410\u041d\u00bb<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/izvestiyakbncran.ru\/index.php\/en\/28-3-11-en\/\" \/>\n<meta property=\"og:locale\" content=\"ru_RU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"28.3.11 En - \u0418\u0417\u0412\u0415\u0421\u0422\u0418\u042f \u041a\u0410\u0411\u0410\u0420\u0414\u0418\u041d\u041e-\u0411\u0410\u041b\u041a\u0410\u0420\u0421\u041a\u041e\u0413\u041e \u041d\u0410\u0423\u0427\u041d\u041e\u0413\u041e \u0426\u0415\u041d\u0422\u0420\u0410 \u0420\u0410\u041d\u00bb\" \/>\n<meta property=\"og:description\" content=\"Impact of Lignohumate antidote application timing on the amino acid composition of winter wheat grain during chemical weeding L.A. 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